Anti-Human KLHL3 Polyclonal Antibody (HA118014)

Name: Anti-Human KLHL3 Polyclonal Antibody
Brand: abinScience
SKU: HA118014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HA118014

Description

Anti-Human KLHL3 Polyclonal Antibody (HA118014) is a rabbit polyclonal antibody detecting KLHL3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human KLHL3 (Thr27-Lys292).

Target

Kelch-like protein 3, KLHL3, KIAA1129

Purification

Purified by antigen affinity column.

Accession

Q9UH77

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Kelch-like protein 3 (KLHL3) is a ~64 kDa protein. Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex acts by mediating ubiquitination and degradation of WNK1 and WNK4, two activators of Na-Cl cotransporter SLC12A3/NCC in distal convoluted tubule cells of kidney, thereby regulating NaCl reabsorption. The BCR(KLHL3) complex also mediates ubiquitination and degradation of WNK3. The BCR(KLHL3) complex also mediates ubiquitination of CLDN8, a tight-junction protein required for paracellular chloride transport in the kidney, leading to its degradation.

1. Furukawa, M. et al. (2003) Nature cell biology 5, 1001-7. PMID: 14528312

2. Louis-Dit-Picard, H. et al. (2012) Nature genetics 44, 456-60, S1-3. PMID: 22406640

3. Ohta, A. et al. (2013) The Biochemical journal 451, 111-22. PMID: 23387299

4. Wakabayashi, M. et al. (2013) Cell reports 3, 858-68. PMID: 23453970

5. Shibata, S. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 7838-43. PMID: 23576762

6. Wu, G. et al. (2013) FEBS letters 587, 1717-22. PMID: 23665031

7. Shibata, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 15556-61. PMID: 25313067

8. Etges, A. et al. (2022) Nephron 146, 418-428. PMID: 35093948

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-KLHL3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse liver lysate

References

Datasheet

Anti-Human KLHL3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human KLHL3 Polyclonal Antibody (HA118014)

Overview

Images

References

Datasheet

SDS

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