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| カタログ番号 | HY349012 |
|---|---|
| 説明 |
Recombinant Human LCAT Protein, N-His (HY349012) expressed in E. coli, spanning Phe25-Glu440. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P04180 |
| タンパク質長 | Phe25-Glu440 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 49.38 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Platelet-activating factor acetylhydrolase, Phosphatidylcholine-sterol acyltransferase, Phospholipid-cholesterol acyltransferase, PAF acetylhydrolase, LCAT, 1-alkyl-2-acetylglycerophosphocholine esterase, Lecithin-cholesterol acyltransferase |
| 背景 | Phosphatidylcholine-sterol acyltransferase (LCAT) is a ~49 kDa protein. Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. 1. Kosek, AB. et al. (1999) Biochemical and biophysical research communications 258, 548-51. PMID: 10329423 2. Hirsch-Reinshagen, V. et al. (2009) Journal of lipid research 50, 885-93. PMID: 19065001 3. Piper, DE. et al. (2015) Journal of lipid research 56, 1711-9. PMID: 26195816 4. Subbaiah, PV. et al. (1996) Journal of lipid research 37, 113-22. PMID: 8820107 5. Clay, MA. et al. (2000) The Journal of biological chemistry 275, 9019-25. PMID: 10722751 6. Liu, M. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 6035-9. PMID: 8016111 7. La Marca, V. et al. (2014) Journal of neurochemistry 130, 97-108. PMID: 24620755 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human LCAT Protein, N-His
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