Human LETM1 Recombinant Protein (N-His) (HF870012)

Name: Human LETM1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF870012
Price: 211 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HF870012
Descripción	Recombinant Human LETM1 Protein, N-His (HF870012) expressed in E. coli, spanning Leu231-Thr426. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	O95202
Longitud de proteína	Leu231-Thr426
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	24.90 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	LETM1, Leucine zipper-EF-hand-containing transmembrane protein 1, Mitochondrial proton/calcium exchanger protein
Fondo	Mitochondrial proton/calcium exchanger protein (LETM1) is a ~83 kDa protein. Plays an important role in maintenance of mitochondrial morphology and in mediating either calcium or potassium/proton antiport. Mediates proton-dependent calcium efflux from mitochondrion. Also functions as an electroneutral mitochondrial proton/potassium exchanger. Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. 1. Tamai, S. et al. (2008) Journal of cell science 121, 2588-600. PMID: 18628306 2. Jiang, D. et al. (2009) Science (New York, N.Y.) 326, 144-7. PMID: 19797662 3. Tsai, MF. et al. (2014) The Journal of general physiology 143, 67-73. PMID: 24344246 4. De Marchi, U. et al. (2014) The Journal of biological chemistry 289, 20377-85. PMID: 24898248 5. Huang, E. et al. (2017) Nature communications 8, 1399. PMID: 29123128 6. Nakamura, S. et al. (2020) Communications biology 3, 99. PMID: 32139798 7. Kaiyrzhanov, R. et al. (2022) American journal of human genetics 109, 1692-1712. PMID: 36055214 8. Austin, S. et al. (2022) EMBO reports 23, e54978. PMID: 36321428
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human LETM1 Protein, N-His

Referencias

Datasheet

Human LETM1 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo