Human LOXL2 Recombinant Protein (C-His) (HP610011)

Name: Human LOXL2 Recombinant Protein (C-His)
Brand: abinScience
SKU: HP610011
Price: 298 USD
Availability: InStock

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50ug
100ug
1mg

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Vista general

Número de catálogo	HP610011
Descripción	Recombinant Human LOXL2 Protein, C-His (HP610011) expressed in Mammalian Cells, spanning Met1-Gln774. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	Mammalian cells
Número de acceso	Q9Y4K0
Longitud de proteína	Met1-Gln774
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Peso molecular predicho	87.69 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	LOXL2, Lysyl oxidase-related protein WS9-14, Lysyl oxidase homolog 2, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2
Fondo	Lysyl oxidase homolog 2 (LOXL2) is a ~86 kDa protein. Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription. LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2. 1. Herranz, N. et al. (2016) The FEBS journal 283, 4263-4273. PMID: 27735137 2. Iturbide, A. et al. (2015) Molecular cell 58, 755-66. PMID: 25959397 3. Peinado, H. et al. (2005) The EMBO journal 24, 3446-58. PMID: 16096638 4. Cuevas, EP. et al. (2014) Biology open 3, 129-37. PMID: 24414204 5. Millanes-Romero, A. et al. (2013) Molecular cell 52, 746-57. PMID: 24239292 6. Cuevas, EP. et al. (2017) Scientific reports 7, 44988. PMID: 28332555
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human LOXL2 Protein, C-His
Bioactivity

Detects Simtuzumab in indirect ELISAs.

Referencias

Datasheet

Human LOXL2 Recombinant Protein (C-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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