Anti-Human LOXL2 Reference Antibody (Simtuzumab, RUO) (HP610016)

Name: Anti-Human LOXL2 Reference Antibody (Simtuzumab, RUO)
Brand: abinScience
SKU: HP610016
Price: 176 USD
Availability: InStock

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Overview

Catalog No.	HP610016
Description	Simtuzumab (HP610016) is a research-grade recombinant antibody targeting LOXL2. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG4-kappa
Expression system	Mammalian cells
Clonality	Monoclonal
Target	LOXL2, Lysyl oxidase-related protein WS9-14, Lysyl oxidase homolog 2, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9Y4K0
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	AB0024, GS-6624, 1318075-13-6
Background	Lysyl oxidase homolog 2 (LOXL2) is a ~86 kDa protein. Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription. LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2. 1. Herranz, N. et al. (2016) The FEBS journal 283, 4263-4273. PMID: 27735137 2. Iturbide, A. et al. (2015) Molecular cell 58, 755-66. PMID: 25959397 3. Peinado, H. et al. (2005) The EMBO journal 24, 3446-58. PMID: 16096638 4. Cuevas, EP. et al. (2014) Biology open 3, 129-37. PMID: 24414204 5. Millanes-Romero, A. et al. (2013) Molecular cell 52, 746-57. PMID: 24239292 6. Cuevas, EP. et al. (2017) Scientific reports 7, 44988. PMID: 28332555
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Simtuzumab.
Bioactivity

Detects LOXL2 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Simtuzumab

References

Datasheet

Anti-Human LOXL2 Reference Antibody (Simtuzumab, RUO).pdf

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Recombinant Proteins

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Anti-Human LOXL2 Reference Antibody (Simtuzumab, RUO) (HP610016)

Overview

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References

Datasheet

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