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| カタログ番号 | HC293011 |
|---|---|
| 説明 |
Recombinant Human LpPLA2/PLA2G7/PAF-AH Protein, C-His (HC293011) expressed in Mammalian Cells. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | Mammalian cells |
| アクセッション番号 | Q13093 |
| タンパク質長 | Met1-Asn441 (Val379Ala) |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 51.02 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Platelet-activating factor acetylhydrolase, PAF acetylhydrolase, 3.1.1.47, 1-alkyl-2-acetylglycerophosphocholine esterase, 2-acetyl-1-alkylglycerophosphocholine esterase, Group-VIIA phospholipase A2, gVIIA-PLA2, LDL-associated phospholipase A2, LDL-PLA(2), PAF 2-acylhydrolase, PLA2G7, PAFAH |
| 背景 | Platelet-activating factor acetylhydrolase (Lp-PLA2/PLA2G7) is a ~50 kDa protein. Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. 1. Stafforini, DM. et al. (1999) The Journal of biological chemistry 274, 7018-24. PMID: 10066756 2. Stafforini, DM. et al. (2006) The Journal of biological chemistry 281, 4616-23. PMID: 16371369 3. Kriska, T. et al. (2007) The Journal of biological chemistry 282, 100-8. PMID: 17090529 4. Stremler, KE. et al. (1991) The Journal of biological chemistry 266, 11095-103. PMID: 2040620 5. Tjoelker, LW. et al. (1995) Nature 374, 549-53. PMID: 7700381 6. Tew, DG. et al. (1996) Arteriosclerosis, thrombosis, and vascular biology 16, 591-9. PMID: 8624782 7. Min, JH. et al. (1999) Biochemistry 38, 12935-42. PMID: 10504265 9. Gardner, AA. et al. (2008) The Journal of biological chemistry 283, 17099-106. PMID: 18434304 |
| 注意事項 | For research use only. |
SDS-PAGE for Human LpPLA2/PLA2G7/PAF-AH Recombinant Protein (C-His)
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