ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Homo sapiens (Human)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
36 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
MAP kinase 8, JNK1, SAPK1C, Stress-activated protein kinase 1c, Mitogen-activated protein kinase 8, SAPK1, c-Jun N-terminal kinase 1, SAPK1c, MAPK8, Stress-activated protein kinase JNK1, PRKM8, JNK-46, MAPK 8
Contexte
Mitogen-activated protein kinase 8 (MAPK8) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.
1. Song, N. et al. (2017) Molecular cell 68, 185-197.e6. PMID: 28943315
2. Murata, T. et al. (2008) Analytical biochemistry 376, 115-21. PMID: 18307971
3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198
4. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637
5. Deng, H. et al. (2011) The international journal of biochemistry & cell biology 43, 409-15. PMID: 21095239
6. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871
7. Hu, Y. et al. (2006) Molecular and cellular biology 26, 3282-94. PMID: 16581800
8. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730
9. Nasrin, N. et al. (2009) PloS one 4, e8414. PMID: 20027304
10. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Human MAPK8 Recombinant Protein (N-His)
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