Anti-MAPK8 Polyclonal Antibody (HW707014)

Name: Anti-MAPK8 Polyclonal Antibody
Brand: abinScience
SKU: HW707014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HW707014

Description

Anti-MAPK8 Polyclonal Antibody (HW707014) is a rabbit polyclonal antibody detecting MAPK8 in ELISA, IHC, WB. Suitable for Cyprinus carpio, Danio rerio, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAPK8 (Tyr26-Ile321).

Target

MAP kinase 8, JNK1, SAPK1C, Stress-activated protein kinase 1c, Mitogen-activated protein kinase 8, SAPK1, c-Jun N-terminal kinase 1, SAPK1c, MAPK8, Stress-activated protein kinase JNK1, PRKM8, JNK-46, MAPK 8

Purification

Purified by antigen affinity column.

Accession

P45983

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitogen-activated protein kinase 8 (MAPK8) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.

1. Song, N. et al. (2017) Molecular cell 68, 185-197.e6. PMID: 28943315

2. Murata, T. et al. (2008) Analytical biochemistry 376, 115-21. PMID: 18307971

3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198

4. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637

5. Deng, H. et al. (2011) The international journal of biochemistry & cell biology 43, 409-15. PMID: 21095239

6. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

7. Hu, Y. et al. (2006) Molecular and cellular biology 26, 3282-94. PMID: 16581800

8. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

9. Nasrin, N. et al. (2009) PloS one 4, e8414. PMID: 20027304

10. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MAPK8 polyclonal antibody at 1ug/mL on recombinant MAPK8 (Catalog No. HW707012).
Western blot

Western blot analysis was performed using anti-JNK polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate

References

Datasheet

Anti-MAPK8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-MAPK8 Polyclonal Antibody (HW707014)

Overview

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References

Datasheet

SDS

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