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| カタログ番号 | HW530012 |
|---|---|
| 説明 |
Recombinant Human MAPK9 Protein, N-His (HW530012) expressed in E. coli, spanning Tyr26-Ile321. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P45984 |
| タンパク質長 | Tyr26-Ile321 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 36.28 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Mitogen-activated protein kinase 9, MAPK 9, Stress-activated protein kinase 1a, SAPK1A, MAP kinase 9, SAPK1a, Stress-activated protein kinase JNK2, PRKM9, JNK-55, MAPK9, JNK2, c-Jun N-terminal kinase 2 |
| 背景 | Mitogen-activated protein kinase 9 (MAPK9) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. 1. De Graeve, F. et al. (1999) Oncogene 18, 3491-500. PMID: 10376527 2. Mayer, C. et al. (2005) Genes & development 19, 933-41. PMID: 15805466 3. Oleinik, NV. et al. (2007) Oncogene 26, 7222-30. PMID: 17525747 4. Hu, D. et al. (2009) PloS one 4, e6640. PMID: 19675674 5. Samak, G. et al. (2010) American journal of physiology. Gastrointestinal and liver physiology 299, G572-84. PMID: 20595622 6. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637 7. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692 8. Yu, F. et al. (2021) Nucleic acids research 49, 5779-5797. PMID: 34048572 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human MAPK9 Protein, N-His
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