Anti-MAPK9 Polyclonal Antibody (HW530014)

Name: Anti-MAPK9 Polyclonal Antibody
Brand: abinScience
SKU: HW530014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HW530014

Description

Anti-MAPK9 Polyclonal Antibody (HW530014) is a rabbit polyclonal antibody detecting MAPK9 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, Rat, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAPK9 (Tyr26-Ile321).

Target

Mitogen-activated protein kinase 9, MAPK 9, Stress-activated protein kinase 1a, SAPK1A, MAP kinase 9, SAPK1a, Stress-activated protein kinase JNK2, PRKM9, JNK-55, MAPK9, JNK2, c-Jun N-terminal kinase 2

Purification

Purified by antigen affinity column.

Accession

P45984

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitogen-activated protein kinase 9 (MAPK9) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3.

1. De Graeve, F. et al. (1999) Oncogene 18, 3491-500. PMID: 10376527

2. Mayer, C. et al. (2005) Genes & development 19, 933-41. PMID: 15805466

3. Oleinik, NV. et al. (2007) Oncogene 26, 7222-30. PMID: 17525747

4. Hu, D. et al. (2009) PloS one 4, e6640. PMID: 19675674

5. Samak, G. et al. (2010) American journal of physiology. Gastrointestinal and liver physiology 299, G572-84. PMID: 20595622

6. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637

7. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692

8. Yu, F. et al. (2021) Nucleic acids research 49, 5779-5797. PMID: 34048572

Note

For research use only.

Images

References

Datasheet

Anti-MAPK9 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-MAPK9 Polyclonal Antibody (HW530014)

Overview

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References

Datasheet

SDS

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