Human MAPRE1 Recombinant Protein (N-His) (HS950012)

Name: Human MAPRE1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HS950012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HS950012
Descripción	Recombinant Human MAPRE1 Protein, N-His (HS950012) expressed in E. coli, spanning Ala2-Tyr268. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q15691
Longitud de proteína	Ala2-Tyr268
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	32.16 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	EB1, Microtubule-associated protein RP/EB family member 1, MAPRE1, APC-binding protein EB1, End-binding protein 1
Fondo	Microtubule-associated protein RP/EB family member 1 (MAPRE1) is a ~29 kDa protein. Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains. Promotes cytoplasmic microtubule nucleation and elongation. Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation. Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers). 1. Askham, JM. et al. (2002) Molecular biology of the cell 13, 3627-45. PMID: 12388762 2. Hayashi, I. et al. (2005) Molecular cell 19, 449-60. PMID: 16109370 3. Honnappa, S. et al. (2009) Cell 138, 366-76. PMID: 19632184 4. van der Vaart, B. et al. (2011) The Journal of cell biology 193, 1083-99. PMID: 21646404 5. Xia, P. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 16564-9. PMID: 23001180 6. Wei, J. et al. (2017) FEBS letters 591, 2379-2393. PMID: 28726242 7. Yang, C. et al. (2017) The Journal of cell biology 216, 3179-3198. PMID: 28814570 8. Song, X. et al. (2021) Nature chemical biology 17, 1314-1323. PMID: 34608293 9. Radhakrishnan, RM. et al. (2023) The Journal of biological chemistry 299, 102853. PMID: 36592928
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human MAPRE1 Protein, N-His

Referencias

Datasheet

Human MAPRE1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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