Anti-MAPRE1 Polyclonal Antibody (HS950014)

Name: Anti-MAPRE1 Polyclonal Antibody
Brand: abinScience
SKU: HS950014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HS950014

Description

Anti-MAPRE1 Polyclonal Antibody (HS950014) is a rabbit polyclonal antibody detecting MAPRE1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Coturnix coturnix.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAPRE1 (Ala2-Tyr268).

Target

EB1, Microtubule-associated protein RP/EB family member 1, MAPRE1, APC-binding protein EB1, End-binding protein 1

Purification

Purified by antigen affinity column.

Accession

Q15691

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Microtubule-associated protein RP/EB family member 1 (MAPRE1) is a ~29 kDa protein. Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Recruits other +TIP proteins to microtubules by binding to a conserved Ser-X-Leu-Pro (SXLP) motif in their polypeptide chains. Promotes cytoplasmic microtubule nucleation and elongation. Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation. Assists chromosome alignment in metaphase by recruiting the SKA complex to the spindle and stabilizing its interactions with microtubule bundles (K-fibers).

1. Askham, JM. et al. (2002) Molecular biology of the cell 13, 3627-45. PMID: 12388762

2. Hayashi, I. et al. (2005) Molecular cell 19, 449-60. PMID: 16109370

3. Honnappa, S. et al. (2009) Cell 138, 366-76. PMID: 19632184

4. van der Vaart, B. et al. (2011) The Journal of cell biology 193, 1083-99. PMID: 21646404

5. Xia, P. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 16564-9. PMID: 23001180

6. Wei, J. et al. (2017) FEBS letters 591, 2379-2393. PMID: 28726242

7. Yang, C. et al. (2017) The Journal of cell biology 216, 3179-3198. PMID: 28814570

8. Song, X. et al. (2021) Nature chemical biology 17, 1314-1323. PMID: 34608293

9. Radhakrishnan, RM. et al. (2023) The Journal of biological chemistry 299, 102853. PMID: 36592928

Note

For research use only.

Images

References

Datasheet

Anti-MAPRE1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MAPRE1 Polyclonal Antibody (HS950014)

Overview

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References

Datasheet

SDS

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