Human Tau Recombinant Protein (C-His) (HY086012)

Name: Human Tau Recombinant Protein (C-His)
Brand: abinScience
SKU: HY086012
Price: 211 USD
Availability: InStock

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Aviso · Catálogo HY086012 ha sido descontinuado.

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1mg

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Vista general

Número de catálogo	HY086012
Descripción	Recombinant Human MAPT/Tau/PHF-tau Protein, C-His (HY086012) expressed in E. coli, spanning Met1-Leu758. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P10636
Longitud de proteína	Met1-Leu758
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Aplicaciones probadas	WB
Objetivo	MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	80.23 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU
Fondo	Microtubule-associated protein tau (MAPT) is a ~78 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. 1. Yoshida, H. et al. (2012) Journal of neurochemistry 120, 165-76. PMID: 21985311 2. Sandberg, A. et al. (2020) Neurobiology of disease 146, 105079. PMID: 32961270
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human MAPT/Tau/PHF-tau Protein, C-His
Bioactivity

Detects Gosuranemab in indirect ELISAs.
Bioactivity

Detects Zagotenemab in indirect ELISAs.
Bioactivity

Detects Tilavonemab in indirect ELISAs.
Western blot

Detected by Anti-MAPT/Tau/PHF-tau monoclonal antibody (Catalog No: HY086013)
Bioactivity

Detected by Research Grade Anti-Human MAPT/Tau/PHF-tau (DC8E8) (Catalog No: HY086013) in indirect ELISA
Bioactivity

Detected by 2C5 (Catalog No: HY086243) in indirect ELISA.
Bioactivity

Detected by SAA2138 (Catalog No: HY086253) in indirect ELISA.