Research Grade Anti-Human MAPT/Tau/PHF-tau (DC8E8) (HY086013)

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50ug
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1mg

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Overview
Images
References
Datasheet

Overview

Catalog No.

HY086013

Description

Anti-Human MAPT/Tau/PHF-tau (DC8E8) (HY086013) is a research-grade recombinant antibody targeting MAPTL. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Species reactivity

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Humanized

Isotype

IgG4-kappa

Clone ID

DC8E8

Expression system

Mammalian Cells

Clonality

Monoclonal

Target

MAPTL, Paired helical filament-tau, MTBT1, Microtubule-associated protein tau, TAU, Neurofibrillary tangle protein, MAPT, PHF-tau

Endotoxin level

Please contact the lab for this information.

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P10636

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.

Background

Microtubule-associated protein tau (MAPT) is a ~78 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

1. Yoshida, H. et al. (2012) Journal of neurochemistry 120, 165-76. PMID: 21985311

2. Sandberg, A. et al. (2020) Neurobiology of disease 146, 105079. PMID: 32961270

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human MAPT/Tau/PHF-tau (DC8E8).
Western blot

Western blot analysis was performed using anti-MAPT/Tau/PHF-tau monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human MAPT/Tau/PHF-tau (Catalog No: HY086012)
Lane 2: negative control
Bioactivity

Detects recombinant human MAPT/Tau/PHF-tau (Catalog No: HY086012) in indirect ELISA