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Human MCM3AP Recombinant Protein (N-His) (HD528012)

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概要
カタログ番号HD528012
説明
Recombinant Human MCM3AP Protein, N-His (HD528012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
発現システムE. coli
アクセッション番号O60318
タンパク質長Thr602-Gly969
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Homo sapiens (Human)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名80 kDa MCM3-associated protein, EC:2.3.1.-, EC:2.3.1.48, GANP, Germinal-center associated nuclear protein, KIAA0572, MAP80, MCM3 acetylating protein, MCM3 acetyltransferase, MCM3AP
背景

Germinal-center associated nuclear protein is a ~218 kDa protein. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores. Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations.

1. Wickramasinghe, VO. et al. (2010) Current biology : CB 20, 25-31. PMID: 20005110
2. Okamoto, N. et al. (2010) Genes to cells : devoted to molecular & cellular mechanisms 15, 471-84. PMID: 20384790
3. Jani, D. et al. (2012) Nucleic acids research 40, 4562-73. PMID: 22307388
4. Umlauf, D. et al. (2013) Journal of cell science 126, 2656-67. PMID: 23591820
5. Singh, SK. et al. (2013) Nature communications 4, 1830. PMID: 23652018
注意事項 For research use only
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Stock Solution
C₁ (Stock Conc.)
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Working Solution
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