Human MIF Recombinant Protein (N-His) (HB816012)

Name: Human MIF Recombinant Protein (N-His)
Brand: abinScience
SKU: HB816012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HB816012
Описание	Recombinant Human MIF/GIF Protein, N-His (HB816012) expressed in E. coli, spanning Met1-Ala115. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P14174
Длина белка	Met1-Ala115
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	14.64 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	MIF, L-dopachrome isomerase, L-dopachrome tautomerase, GIF, Macrophage migration inhibitory factor, MMIF, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GLIF
Фон	Macrophage migration inhibitory factor (MIF/GIF) is a ~12 kDa protein. Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. 1. Oddo, M. et al. (2005) Infection and immunity 73, 3783-6. PMID: 15908412 2. Emonts, M. et al. (2007) Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 44, 1321-8. PMID: 17443469 3. Fan, C. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10994-9. PMID: 23776208 4. Tan, TH. et al. (2001) The Biochemical journal 357, 373-83. PMID: 11439086
Примечание	For research use only.