Human MMP14 Recombinant Protein (C-His) (HW299022)

Name: Human MMP14 Recombinant Protein (C-His)
Brand: abinScience
SKU: HW299022
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Datasheet
SDS

Vista general

Número de catálogo	HW299022
Descripción	Recombinant Human MMP14 Protein, C-His (HW299022) expressed in E. coli, spanning Ala21-Ser287. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P50281
Longitud de proteína	Ala21-Ser287
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	31.12 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP
Fondo	Matrix metalloproteinase-14 (MMP14) is a ~65 kDa protein. Endopeptidase that degrades various components of the extracellular matrix such as collagen. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. Activates progelatinase A/MMP2, thereby acting as a positive regulator of cell growth and migration. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. May be involved in actin cytoskeleton reorganization by cleaving PTK7. 1. Sato, H. et al. (1994) Nature 370, 61-5. PMID: 8015608 2. Gu, G. et al. (2012) Journal of cellular biochemistry 113, 1013-21. PMID: 22065321 3. Noda, K. et al. (2003) Investigative ophthalmology & visual science 44, 2163-70. PMID: 12714657 4. Golubkov, VS. et al. (2010) The Journal of biological chemistry 285, 35740-9. PMID: 20837484 5. Jin, G. et al. (2011) The EMBO journal 30, 2281-93. PMID: 21572390 6. Cork, SM. et al. (2012) Oncogene 31, 5144-52. PMID: 22330140 7. Chow, CFW. et al. (2022) Nature metabolism 4, 203-212. PMID: 35177851
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human MMP14 Protein, C-His
Bioactivity

Detects Human MMP14 Antibody (LEM-2/15) in indirect ELISAs.
Western blot

Detected by Anti-MMP14 monoclonal antibody (Catalog No: HW299013)

Referencias

Datasheet

Human MMP14 Recombinant Protein (C-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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