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Anti-Human MMP14 Antibody (LEM-2/15) (HW299013)

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  • Datasheet

Overview
Catalog No.HW299013
Species reactivityHomo sapiens (Human)
ApplicationsBlocking, ELISA, ICC, IF, FCM, WB
Host speciesMouse
IsotypeIgG1, kappa
Clone IDLEM-2/15
Clonality Monoclonal
Target MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P50281
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Bioactivity

    Detects MMP14 in indirect ELISAs.

  • SDS-PAGE

    SDS-PAGE for Anti-Human MMP14 Antibody (LEM-2/15).

  • Flow Cytometry

    Flow-cytometry using anti-human MMP14 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 antibody monoclonal antibody (Catalog # HW299013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human MMP14 (Catalog No: HW299022)
    Lane 2: negative control

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