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| カタログ番号 | HT177012 |
|---|---|
| 説明 |
Recombinant Human NDC80/HEC1 Protein, N-His (HT177012) expressed in E. coli, spanning Pro290-Glu642. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O14777 |
| タンパク質長 | Pro290-Glu642 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | HEC1, Kinetochore protein Hec1, HsHec1, HEC, Kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80, Highly expressed in cancer protein, Retinoblastoma-associated protein HEC, KNTC2 |
| 背景 | Kinetochore protein NDC80 homolog (HEC1) is a ~73 kDa protein. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. 1. Martin-Lluesma, S. et al. (2002) Science (New York, N.Y.) 297, 2267-70. PMID: 12351790 2. DeLuca, JG. et al. (2003) Current biology : CB 13, 2103-9. PMID: 14654001 3. Bharadwaj, R. et al. (2004) The Journal of biological chemistry 279, 13076-85. PMID: 14699129 4. Joseph, J. et al. (2004) Current biology : CB 14, 611-7. PMID: 15062103 5. Stucke, VM. et al. (2004) Chromosoma 113, 1-15. PMID: 15235793 6. Meraldi, P. et al. (2004) Developmental cell 7, 45-60. PMID: 15239953 7. DeLuca, JG. et al. (2005) Molecular biology of the cell 16, 519-31. PMID: 15548592 8. Lin, YT. et al. (2006) Oncogene 25, 6901-14. PMID: 16732327 9. Zhao, G. et al. (2019) The Journal of biological chemistry 294, 576-592. PMID: 30409912 10. Chen, Y. et al. (1997) Molecular and cellular biology 17, 6049-56. PMID: 9315664 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human NDC80/HEC1 Protein, N-His
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