Anti-NDC80/HEC1 Polyclonal Antibody (HT177014)

Name: Anti-NDC80/HEC1 Polyclonal Antibody
Brand: abinScience
SKU: HT177014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT177014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NDC80/HEC1 (Pro290-Glu642).

Target

HEC1, Kinetochore protein Hec1, HsHec1, HEC, Kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80, Highly expressed in cancer protein, Retinoblastoma-associated protein HEC, KNTC2

Purification

Purified by antigen affinity column.

Accession

O14777

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Kinetochore protein NDC80 homolog (HEC1) is a ~73 kDa protein. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.

1. Martin-Lluesma, S. et al. (2002) Science (New York, N.Y.) 297, 2267-70. PMID: 12351790

2. DeLuca, JG. et al. (2003) Current biology : CB 13, 2103-9. PMID: 14654001

3. Bharadwaj, R. et al. (2004) The Journal of biological chemistry 279, 13076-85. PMID: 14699129

4. Joseph, J. et al. (2004) Current biology : CB 14, 611-7. PMID: 15062103

5. Stucke, VM. et al. (2004) Chromosoma 113, 1-15. PMID: 15235793

6. Meraldi, P. et al. (2004) Developmental cell 7, 45-60. PMID: 15239953

7. DeLuca, JG. et al. (2005) Molecular biology of the cell 16, 519-31. PMID: 15548592

8. Lin, YT. et al. (2006) Oncogene 25, 6901-14. PMID: 16732327

9. Zhao, G. et al. (2019) The Journal of biological chemistry 294, 576-592. PMID: 30409912

10. Chen, Y. et al. (1997) Molecular and cellular biology 17, 6049-56. PMID: 9315664

Note

For research use only.

Images

References

Datasheet

Anti-NDC80/HEC1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NDC80/HEC1 Polyclonal Antibody (HT177014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us