Human NDOR1 Recombinant Protein (N-His) (HP735012)

Name: Human NDOR1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP735012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HP735012
Descripción	Recombinant Human NDOR1 Protein, N-His (HP735012) expressed in E. coli, spanning Met1-Leu154. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9UHB4
Longitud de proteína	Met1-Leu154
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	19.37 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	NR1, NDOR1, NADPH-dependent diflavin oxidoreductase 1, NADPH-dependent FMN and FAD-containing oxidoreductase, Novel reductase 1
Fondo	NADPH-dependent diflavin oxidoreductase 1 (NDOR1) is a ~66 kDa protein. NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair. In vitro can fully activate methionine synthase/MTR in the presence of soluble cytochrome b5/CYB5A. 1. Paine, MJ. et al. (2000) The Journal of biological chemistry 275, 1471-8. PMID: 10625700 2. Finn, RD. et al. (2005) Pharmacogenetics and genomics 15, 381-6. PMID: 15900210 3. Netz, DJ. et al. (2010) Nature chemical biology 6, 758-65. PMID: 20802492 4. Banci, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 7136-41. PMID: 23596212 5. Camponeschi, F. et al. (2017) Journal of the American Chemical Society 139, 9479-9482. PMID: 28648056
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human NDOR1 Protein, N-His

Referencias

Datasheet

Human NDOR1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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