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Anti-Human NDOR1 Polyclonal Antibody (HP735014)

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Overview
Catalog No.HP735014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NDOR1 (Met1-Leu154).
Target NR1, NDOR1, NADPH-dependent diflavin oxidoreductase 1, NADPH-dependent FMN and FAD-containing oxidoreductase, Novel reductase 1
Purification Purified by antigen affinity column.
Accession Q9UHB4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

NADPH-dependent diflavin oxidoreductase 1 (NDOR1) is a ~66 kDa protein. NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair. In vitro can fully activate methionine synthase/MTR in the presence of soluble cytochrome b5/CYB5A.

1. Paine, MJ. et al. (2000) The Journal of biological chemistry 275, 1471-8. PMID: 10625700
2. Finn, RD. et al. (2005) Pharmacogenetics and genomics 15, 381-6. PMID: 15900210
3. Netz, DJ. et al. (2010) Nature chemical biology 6, 758-65. PMID: 20802492
4. Banci, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 7136-41. PMID: 23596212
5. Camponeschi, F. et al. (2017) Journal of the American Chemical Society 139, 9479-9482. PMID: 28648056
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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Anti-Human NDOR1 Polyclonal Antibody

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