中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC254012 |
|---|---|
| 説明 |
Recombinant Human NEDD8 Protein, N-His-SUMO (HC254012) expressed in E. coli, spanning Leu2-Gln81. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q15843 |
| タンパク質長 | Leu2-Gln81 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 21.28 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | NEDD-8, NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8 |
| 背景 | Ubiquitin-like protein NEDD8 is a ~9 kDa protein. Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. 1. Liakopoulos, D. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 5510-5. PMID: 10318914 2. Hori, T. et al. (1999) Oncogene 18, 6829-34. PMID: 10597293 3. Amir, RE. et al. (2002) The Journal of biological chemistry 277, 23253-9. PMID: 11953428 4. Walden, H. et al. (2003) Molecular cell 12, 1427-37. PMID: 14690597 5. Xirodimas, DP. et al. (2004) Cell 118, 83-97. PMID: 15242646 6. Osaka, F. et al. (1998) Genes & development 12, 2263-8. PMID: 9694792 7. Horn-Ghetko, D. et al. (2024) Nature structural & molecular biology 31, 1083-1094. PMID: 38605244 8. Kokic, G. et al. (2024) Nature structural & molecular biology 31, 536-547. PMID: 38316879 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
