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Anti-Human NEDD8 Polyclonal Antibody (HC254014)

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Overview
Catalog No.HC254014
Description
Anti-Human NEDD8 Polyclonal Antibody (HC254014) is a rabbit polyclonal antibody detecting NEDD8. Suitable for Rat, Mouse, and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NEDD8 (Leu2-Gln81).
Target NEDD-8, NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q15843
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Ubiquitin-like protein NEDD8 is a ~9 kDa protein. Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.

1. Liakopoulos, D. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 5510-5. PMID: 10318914
2. Hori, T. et al. (1999) Oncogene 18, 6829-34. PMID: 10597293
3. Amir, RE. et al. (2002) The Journal of biological chemistry 277, 23253-9. PMID: 11953428
4. Walden, H. et al. (2003) Molecular cell 12, 1427-37. PMID: 14690597
5. Xirodimas, DP. et al. (2004) Cell 118, 83-97. PMID: 15242646
6. Osaka, F. et al. (1998) Genes & development 12, 2263-8. PMID: 9694792
7. Horn-Ghetko, D. et al. (2024) Nature structural & molecular biology 31, 1083-1094. PMID: 38605244
8. Kokic, G. et al. (2024) Nature structural & molecular biology 31, 536-547. PMID: 38316879
Note For research use only
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Datasheet
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Formula
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V₁ (Stock Vol.)
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Working Solution
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Anti-Human NEDD8 Polyclonal Antibody

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