Human NELFE Recombinant Protein (N-His) (HB121022)

Name: Human NELFE Recombinant Protein (N-His)
Brand: abinScience
SKU: HB121022
Price: 211 USD
Availability: InStock

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Número de catálogo	HB121022
Descripción	Recombinant Human NELFE Protein, N-His (HB121022) expressed in E. coli, spanning Ser249-Phe380. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P18615
Longitud de proteína	Ser249-Phe380
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	16.96 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	NELF-E, NELFE, Negative elongation factor E, RD, RDBP, RNA-binding protein RD
Fondo	Negative elongation factor E is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. 1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401 2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882 3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650 4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062 5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858 6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391
Nota	For research use only

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Datasheet

Human NELFE Recombinant Protein (N-His).pdf

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Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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