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| カタログ番号 | HB121022 |
|---|---|
| 説明 |
Recombinant Human NELFE Protein, N-His (HB121022) expressed in E. coli, spanning Ser249-Phe380. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P18615 |
| タンパク質長 | Ser249-Phe380 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 16.96 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | NELF-E, NELFE, Negative elongation factor E, RD, RDBP, RNA-binding protein RD |
| 背景 | Negative elongation factor E is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. 1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401 2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882 3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650 4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062 5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858 6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391 |
| 注意事項 | For research use only |
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