Human NELFE Recombinant Protein (N-His) (HB121022)

Name: Human NELFE Recombinant Protein (N-His)
Brand: abinScience
SKU: HB121022
Price: 211 USD
Availability: InStock

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100ug
1mg

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Обзор

Каталожный номер	HB121022
Описание	Recombinant Human NELFE Protein, N-His (HB121022) expressed in E. coli, spanning Ser249-Phe380. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P18615
Длина белка	Ser249-Phe380
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	16.96 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	NELF-E, NELFE, Negative elongation factor E, RD, RDBP, RNA-binding protein RD
Фон	Negative elongation factor E is a ~43 kDa protein. Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. 1. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401 2. Gibson, BA. et al. (2016) Science (New York, N.Y.) 353, 45-50. PMID: 27256882 3. Yamaguchi, Y. et al. (2002) Molecular and cellular biology 22, 2918-27. PMID: 11940650 4. Narita, T. et al. (2003) Molecular and cellular biology 23, 1863-73. PMID: 12612062 5. Rao, JN. et al. (2008) Biochemistry 47, 3756-61. PMID: 18303858 6. Vos, SM. et al. (2016) eLife 5. PMID: 27282391
Примечание	For research use only

Изображения

Ссылки

Даташит

Human NELFE Recombinant Protein (N-His).pdf

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Formula

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