Human NPC2 Recombinant Protein (N-His) (HX127012)

Name: Human NPC2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX127012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HX127012
Descripción	Recombinant Human NPC2 Protein, N-His (HX127012) expressed in E. coli, spanning Asp31-Leu151. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P61916
Longitud de proteína	Asp31-Leu151
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	15.68 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	HE1, NPC intracellular cholesterol transporter 2, He1, Epididymal secretory protein E1, Human epididymis-specific protein 1, NPC2, Niemann-Pick disease type C2 protein
Fondo	NPC intracellular cholesterol transporter 2 (NPC2) is a ~16 kDa protein. Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. 1. Naureckiene, S. et al. (2000) Science (New York, N.Y.) 290, 2298-301. PMID: 11125141 2. Chikh, K. et al. (2005) Human mutation 26, 20-8. PMID: 15937921 3. Liou, HL. et al. (2006) The Journal of biological chemistry 281, 36710-23. PMID: 17018531 4. Infante, RE. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15287-92. PMID: 18772377 5. Berzina, Z. et al. (2018) Chemistry and physics of lipids 213, 48-61. PMID: 29580834 6. Gong, X. et al. (2016) Cell 165, 1467-1478. PMID: 27238017 7. Xu, Z. et al. (2008) Biochemistry 47, 11134-43. PMID: 18823126
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human NPC2 Protein, N-His

Referencias

Datasheet

Human NPC2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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