Anti-Human NPC2 Polyclonal Antibody (HX127014)

Name: Anti-Human NPC2 Polyclonal Antibody
Brand: abinScience
SKU: HX127014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HX127014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human NPC2 (Asp31-Leu151).

Target

HE1, NPC intracellular cholesterol transporter 2, He1, Epididymal secretory protein E1, Human epididymis-specific protein 1, NPC2, Niemann-Pick disease type C2 protein

Purification

Purified by antigen affinity column.

Accession

P61916

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NPC intracellular cholesterol transporter 2 (NPC2) is a ~16 kDa protein. Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol.

1. Naureckiene, S. et al. (2000) Science (New York, N.Y.) 290, 2298-301. PMID: 11125141

2. Chikh, K. et al. (2005) Human mutation 26, 20-8. PMID: 15937921

3. Liou, HL. et al. (2006) The Journal of biological chemistry 281, 36710-23. PMID: 17018531

4. Infante, RE. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15287-92. PMID: 18772377

5. Berzina, Z. et al. (2018) Chemistry and physics of lipids 213, 48-61. PMID: 29580834

6. Gong, X. et al. (2016) Cell 165, 1467-1478. PMID: 27238017

7. Xu, Z. et al. (2008) Biochemistry 47, 11134-43. PMID: 18823126

Note

For research use only.

Images

References

Datasheet

Anti-Human NPC2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human NPC2 Polyclonal Antibody (HX127014)

Overview

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References

Datasheet

SDS

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