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| カタログ番号 | HX127012 |
|---|---|
| 説明 |
Recombinant Human NPC2 Protein, N-His (HX127012) expressed in E. coli, spanning Asp31-Leu151. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P61916 |
| タンパク質長 | Asp31-Leu151 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.68 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | HE1, NPC intracellular cholesterol transporter 2, He1, Epididymal secretory protein E1, Human epididymis-specific protein 1, NPC2, Niemann-Pick disease type C2 protein |
| 背景 | NPC intracellular cholesterol transporter 2 (NPC2) is a ~16 kDa protein. Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. May bind and mobilize cholesterol that is associated with membranes. NPC2 binds cholesterol with a 1:1 stoichiometry. Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol. 1. Naureckiene, S. et al. (2000) Science (New York, N.Y.) 290, 2298-301. PMID: 11125141 2. Chikh, K. et al. (2005) Human mutation 26, 20-8. PMID: 15937921 3. Liou, HL. et al. (2006) The Journal of biological chemistry 281, 36710-23. PMID: 17018531 4. Infante, RE. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15287-92. PMID: 18772377 5. Berzina, Z. et al. (2018) Chemistry and physics of lipids 213, 48-61. PMID: 29580834 6. Gong, X. et al. (2016) Cell 165, 1467-1478. PMID: 27238017 7. Xu, Z. et al. (2008) Biochemistry 47, 11134-43. PMID: 18823126 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human NPC2 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
