Human NPS Recombinant Protein (N-GST) (HY501012)

Name: Human NPS Recombinant Protein (N-GST)
Brand: abinScience
SKU: HY501012
Price: 211 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue	HY501012
Description	Recombinant Human NPS Protein, N-GST (HY501012) expressed in E. coli, spanning Ser29-Lys88. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P0C0P6
Longueur de protéine	Ser29-Lys88
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	33.68 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	NPS, Neuropeptide S
Contexte	Neuropeptide S (NPS) is a ~10 kDa protein. Ligand for NPSR1. Binds to its receptor with nanomolar affinity and initiates a G(q)/GNAQ-dependent phospholipase C-activating signaling pathway. This results in Ca(2+) mobilization from intracellular stores and increased intracellular Ca(2+) levels. In addition to this pathway, NPS binding to its receptor activates cAMP/PKA signal transduction. Finally, both pathways converge to activate ERK1/ERK2 phosphorylation and signaling cascade. 1. Xu, YL. et al. (2004) Neuron 43, 487-97. PMID: 15312648 2. Vendelin, J. et al. (2005) American journal of respiratory cell and molecular biology 33, 262-70. PMID: 15947423 3. Roth, AL. et al. (2006) The Journal of biological chemistry 281, 20809-20816. PMID: 16720571 4. Bernier, V. et al. (2006) The Journal of biological chemistry 281, 24704-12. PMID: 16790440 5. Erdmann, F. et al. (2015) PloS one 10, e0117319. PMID: 25714705 6. Liao, Y. et al. (2016) The Journal of biological chemistry 291, 7505-16. PMID: 26865629
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human NPS Protein, N-GST

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Human NPS Recombinant Protein (N-GST).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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