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Anti-NPS Polyclonal Antibody (HY501014)

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Overview
Catalog No.HY501014
Description
Anti-NPS Polyclonal Antibody (HY501014) is a rabbit polyclonal antibody detecting NPS in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NPS (Ser29-Lys88).
Target NPS, Neuropeptide S
Purification Purified by antigen affinity column.
Accession P0C0P6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Neuropeptide S (NPS) is a ~10 kDa protein. Ligand for NPSR1. Binds to its receptor with nanomolar affinity and initiates a G(q)/GNAQ-dependent phospholipase C-activating signaling pathway. This results in Ca(2+) mobilization from intracellular stores and increased intracellular Ca(2+) levels. In addition to this pathway, NPS binding to its receptor activates cAMP/PKA signal transduction. Finally, both pathways converge to activate ERK1/ERK2 phosphorylation and signaling cascade.

1. Xu, YL. et al. (2004) Neuron 43, 487-97. PMID: 15312648
2. Vendelin, J. et al. (2005) American journal of respiratory cell and molecular biology 33, 262-70. PMID: 15947423
3. Roth, AL. et al. (2006) The Journal of biological chemistry 281, 20809-20816. PMID: 16720571
4. Bernier, V. et al. (2006) The Journal of biological chemistry 281, 24704-12. PMID: 16790440
5. Erdmann, F. et al. (2015) PloS one 10, e0117319. PMID: 25714705
6. Liao, Y. et al. (2016) The Journal of biological chemistry 291, 7505-16. PMID: 26865629
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-NPS Polyclonal Antibody

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