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| カタログ番号 | HV836012 |
|---|---|
| 説明 |
Recombinant Human NR1H4/FXR Protein, N-His (HV836012) expressed in E. coli, spanning Leu211-Gln486. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q96RI1 |
| タンパク質長 | Leu211-Gln486 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 34.5 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Farnesoid X-activated receptor, Farnesol receptor HRR-1, HRR1, RXR-interacting protein 14, BAR, FXR, Bile acid receptor, NR1H4, RIP14, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14 |
| 背景 | Bile acid receptor (NR1H4/FXR) is a ~55 kDa protein. Ligand-activated transcription factor. Receptor for bile acids (BAs) such as chenodeoxycholic acid (CDCA), lithocholic acid, deoxycholic acid (DCA) and allocholic acid (ACA). Plays a essential role in BA homeostasis through the regulation of genes involved in BA synthesis, conjugation and enterohepatic circulation. Also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands. 1. Makishima, M. et al. (1999) Science (New York, N.Y.) 284, 1362-5. PMID: 10334992 2. Parks, DJ. et al. (1999) Science (New York, N.Y.) 284, 1365-8. PMID: 10334993 3. Modica, S. et al. (2010) Nuclear receptor signaling 8, e005. PMID: 21383957 4. Hollman, DA. et al. (2012) Biochimica et biophysica acta 1821, 1443-52. PMID: 22820415 5. Pircher, PC. et al. (2003) The Journal of biological chemistry 278, 27703-11. PMID: 12754200 6. Ananthanarayanan, M. et al. (2004) The Journal of biological chemistry 279, 54348-57. PMID: 15471871 7. Sanyal, S. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 15665-70. PMID: 17895379 8. Grober, J. et al. (1999) The Journal of biological chemistry 274, 29749-54. PMID: 10514450 9. Neimark, E. et al. (2004) Hepatology (Baltimore, Md.) 40, 149-56. PMID: 15239098 10. Landrier, JF. et al. (2006) American journal of physiology. Gastrointestinal and liver physiology 290, G476-85. PMID: 16269519 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human NR1H4/FXR Protein, N-His
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