Anti-NR1H4/FXR Polyclonal Antibody (HV836014)

Name: Anti-NR1H4/FXR Polyclonal Antibody
Brand: abinScience
SKU: HV836014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HV836014

Description

Anti-NR1H4/FXR Polyclonal Antibody (HV836014) is a rabbit polyclonal antibody detecting NR1H4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NR1H4/FXR (Leu211-Gln486).

Target

Farnesoid X-activated receptor, Farnesol receptor HRR-1, HRR1, RXR-interacting protein 14, BAR, FXR, Bile acid receptor, NR1H4, RIP14, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14

Purification

Purified by antigen affinity column.

Accession

Q96RI1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Bile acid receptor (NR1H4/FXR) is a ~55 kDa protein. Ligand-activated transcription factor. Receptor for bile acids (BAs) such as chenodeoxycholic acid (CDCA), lithocholic acid, deoxycholic acid (DCA) and allocholic acid (ACA). Plays a essential role in BA homeostasis through the regulation of genes involved in BA synthesis, conjugation and enterohepatic circulation. Also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands.

1. Makishima, M. et al. (1999) Science (New York, N.Y.) 284, 1362-5. PMID: 10334992

2. Parks, DJ. et al. (1999) Science (New York, N.Y.) 284, 1365-8. PMID: 10334993

3. Modica, S. et al. (2010) Nuclear receptor signaling 8, e005. PMID: 21383957

4. Hollman, DA. et al. (2012) Biochimica et biophysica acta 1821, 1443-52. PMID: 22820415

5. Pircher, PC. et al. (2003) The Journal of biological chemistry 278, 27703-11. PMID: 12754200

6. Ananthanarayanan, M. et al. (2004) The Journal of biological chemistry 279, 54348-57. PMID: 15471871

7. Sanyal, S. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 15665-70. PMID: 17895379

8. Grober, J. et al. (1999) The Journal of biological chemistry 274, 29749-54. PMID: 10514450

9. Neimark, E. et al. (2004) Hepatology (Baltimore, Md.) 40, 149-56. PMID: 15239098

10. Landrier, JF. et al. (2006) American journal of physiology. Gastrointestinal and liver physiology 290, G476-85. PMID: 16269519

Note

For research use only.

Images

References

Datasheet

Anti-NR1H4/FXR Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-NR1H4/FXR Polyclonal Antibody (HV836014)

Overview

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