Human NSUN6 Recombinant Protein (N-His) (HC256012)

Name: Human NSUN6 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC256012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HC256012
Descripción	Recombinant Human NSUN6 Protein, N-His (HC256012) expressed in E. coli, spanning Met1-Thr469. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q8TEA1
Longitud de proteína	Met1-Thr469
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	53.93 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	EC:2.1.1.-, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6, NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
Fondo	tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 is a ~51 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates. 1. Haag, S. et al. (2015) RNA (New York, N.Y.) 21, 1532-43. PMID: 26160102 2. Long, T. et al. (2016) The Journal of biological chemistry 291, 24293-24303. PMID: 27703015 3. Liu, RJ. et al. (2017) Nucleic acids research 45, 6684-6697. PMID: 28531330
Nota	For research use only

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Referencias

Datasheet

Human NSUN6 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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