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Anti-Human NSUN6 Polyclonal Antibody (HC256014)

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Overview
Catalog No.HC256014
Description
Anti-Human NSUN6 Polyclonal Antibody (HC256014) is a rabbit polyclonal antibody detecting NSUN6. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NSUN6 (Met1-Thr469).
Target EC:2.1.1.-, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6, NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q8TEA1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 is a ~51 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.

1. Haag, S. et al. (2015) RNA (New York, N.Y.) 21, 1532-43. PMID: 26160102
2. Long, T. et al. (2016) The Journal of biological chemistry 291, 24293-24303. PMID: 27703015
3. Liu, RJ. et al. (2017) Nucleic acids research 45, 6684-6697. PMID: 28531330
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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