Please ensure Javascript is enabled for purposes of website accessibility
Accueil > Products > Protéines Recombinantes > Autres Protéines

Human NSUN6 Recombinant Protein (N-His) (HC256012)

Prix(USD) :
Spécification :
  • 50ug
  • 100ug
  • 1mg
Nombre :
Contactez-nous
  • Aperçu

  • Images

  • Reférences

  • Fiche technique

  • SDS

Aperçu
Numéro de catalogueHC256012
Description
Recombinant Human NSUN6 Protein, N-His (HC256012) expressed in E. coli, spanning Met1-Thr469. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ8TEA1
Longueur de protéineMet1-Thr469
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 53.93 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsEC:2.1.1.-, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6, NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
Contexte

tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 is a ~51 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.

1. Haag, S. et al. (2015) RNA (New York, N.Y.) 21, 1532-43. PMID: 26160102
2. Long, T. et al. (2016) The Journal of biological chemistry 291, 24293-24303. PMID: 27703015
3. Liu, RJ. et al. (2017) Nucleic acids research 45, 6684-6697. PMID: 28531330
Note For research use only
Images
Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

Contactez-nous pour les devis personnalisés, les demandes en gros et tout autre problème.

Courrier : [email protected]

Liste des distributeurs