Human NSUN6 Recombinant Protein (N-His) (HC256012)

Name: Human NSUN6 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC256012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Обзор

Каталожный номер	HC256012
Описание	Recombinant Human NSUN6 Protein, N-His (HC256012) expressed in E. coli, spanning Met1-Thr469. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q8TEA1
Длина белка	Met1-Thr469
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	53.93 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	EC:2.1.1.-, NOL1/NOP2/Sun and PUA domain-containing protein 1, NOL1/NOP2/Sun domain family member 6, NOPD1, NSUN6, tRNA (cytosine(72)-C(5))-methyltransferase NSUN6
Фон	tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 is a ~51 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates. 1. Haag, S. et al. (2015) RNA (New York, N.Y.) 21, 1532-43. PMID: 26160102 2. Long, T. et al. (2016) The Journal of biological chemistry 291, 24293-24303. PMID: 27703015 3. Liu, RJ. et al. (2017) Nucleic acids research 45, 6684-6697. PMID: 28531330
Примечание	For research use only

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Ссылки

Даташит

Human NSUN6 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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