Human NUPR1 Recombinant Protein (N-His-KSI) (HA758012)

Name: Human NUPR1 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HA758012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HA758012
Description	Recombinant Human NUPR1 Protein, N-His-KSI (HA758012) expressed in E. coli, spanning Ala2-Arg82. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O60356
Longueur de protéine	Ala2-Arg82
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	24.50 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Nuclear protein 1; Candidate of metastasis 1; Protein p8; NUPR1; COM1
Contexte	Nuclear protein 1 is a ~8 kDa protein. Transcription regulator that converts stress signals into a program of gene expression that empowers cells with resistance to the stress induced by a change in their microenvironment. Thereby participates in the regulation of many processes namely cell-cycle, apoptosis, autophagy and DNA repair responses. Controls cell cycle progression and protects cells from genotoxic stress induced by doxorubicin through the complex formation with TP53 and EP300 that binds CDKN1A promoter leading to transcriptional induction of CDKN1A. Protects pancreatic cancer cells from stress-induced cell death by binding the RELB promoter and activating its transcription, leading to IER3 transactivation. Negatively regulates apoptosis through interaction with PTMA. 1. Encinar, JA. et al. (2001) The Journal of biological chemistry 276, 2742-51. PMID: 11056169 2. Hoffmeister, A. et al. (2002) The Journal of biological chemistry 277, 22314-9. PMID: 11940591 3. Malicet, C. et al. (2006) Biochemical and biophysical research communications 339, 284-9. PMID: 16300740 4. Malicet, C. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 2671-6. PMID: 16478804 5. Clark, DW. et al. (2008) Current cancer drug targets 8, 421-30. PMID: 18690848 6. Gironella, M. et al. (2009) Journal of cellular physiology 221, 594-602. PMID: 19650074 7. Sambasivan, R. et al. (2009) Journal of cell science 122, 3481-91. PMID: 19723804 8. Kong, DK. et al. (2010) Molecular biology of the cell 21, 1335-49. PMID: 20181828 9. Hamidi, T. et al. (2012) The Journal of clinical investigation 122, 2092-103. PMID: 22565310 10. Vincent, AJ. et al. (2012) FEBS letters 586, 3429-34. PMID: 22858377
Note	For research use only

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SDS-PAGE for Recombinant Human NUPR1 Protein, N-His-KSI

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Human NUPR1 Recombinant Protein (N-His-KSI).pdf

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