ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>95% as determined by SDS-PAGE.
Peso molecular predicho
86.11 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Protein O-GlcNAcase (OGA) is a ~102 kDa protein. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.
1. Gao, Y. et al. (2001) The Journal of biological chemistry 276, 9838-45. PMID: 11148210
2. Wells, L. et al. (2002) The Journal of biological chemistry 277, 1755-61. PMID: 11788610
3. Li, J. et al. (2010) Biochemistry. Biokhimiia 75, 938-43. PMID: 20673219
4. Schimpl, M. et al. (2012) Chemistry & biology 19, 173-8. PMID: 22365600
5. Rao, FV. et al. (2013) Open biology 3, 130021. PMID: 24088714
6. Li, B. et al. (2017) Nature communications 8, 666. PMID: 28939839
7. Yu, F. et al. (2024) Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73, 5-17. PMID: 37962578
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human OGA Protein, N-His-SUMO
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