Anti-OGA Polyclonal Antibody (HT134014)

Name: Anti-OGA Polyclonal Antibody
Brand: abinScience
SKU: HT134014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HT134014

Description

Anti-OGA Polyclonal Antibody (HT134014) is a rabbit polyclonal antibody detecting OGA in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human OGA (Ser6-Lys240).

Target

OGA, Protein O-GlcNAcase, Meningioma-expressed antigen 5, Beta-N-acetylhexosaminidase, HEXC, N-acetyl-beta-glucosaminidase, KIAA0679, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, MEA5, MGEA5, Beta-hexosaminidase, NCOAT, Beta-N-acetylglucosaminidase, N-acetyl-beta-D-glucosaminidase

Purification

Purified by antigen affinity column.

Accession

O60502

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein O-GlcNAcase (OGA) is a ~102 kDa protein. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.

1. Gao, Y. et al. (2001) The Journal of biological chemistry 276, 9838-45. PMID: 11148210

2. Wells, L. et al. (2002) The Journal of biological chemistry 277, 1755-61. PMID: 11788610

3. Li, J. et al. (2010) Biochemistry. Biokhimiia 75, 938-43. PMID: 20673219

4. Schimpl, M. et al. (2012) Chemistry & biology 19, 173-8. PMID: 22365600

5. Rao, FV. et al. (2013) Open biology 3, 130021. PMID: 24088714

6. Li, B. et al. (2017) Nature communications 8, 666. PMID: 28939839

7. Yu, F. et al. (2024) Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73, 5-17. PMID: 37962578

Note

For research use only.

Images

References

Datasheet

Anti-OGA Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-OGA Polyclonal Antibody (HT134014)

Overview

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References

Datasheet

SDS

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