中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC494012 |
|---|---|
| 説明 |
Recombinant Human PAK1 Protein, N-His (HC494012) expressed in E. coli, spanning Glu252-Cys501. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q13153 |
| タンパク質長 | Glu252-Cys501 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 30.33 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Alpha-PAK, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, PAK1 |
| 背景 | Serine/threonine-protein kinase PAK 1 (PAK1) is a ~60 kDa protein. Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. 1. Zenke, FT. et al. (1999) The Journal of biological chemistry 274, 32565-73. PMID: 10551809 2. Manabe, R. et al. (2002) Journal of cell science 115, 1497-510. PMID: 11896197 3. Slack-Davis, JK. et al. (2003) The Journal of cell biology 162, 281-91. PMID: 12876277 4. Zhou, GL. et al. (2003) Molecular and cellular biology 23, 8058-69. PMID: 14585966 5. Zhou, H. et al. (2005) The Journal of biological chemistry 280, 10624-35. PMID: 15611088 6. Rider, L. et al. (2007) The Journal of biological chemistry 282, 30985-96. PMID: 17726028 7. Mayhew, MW. et al. (2007) Journal of cell science 120, 3911-8. PMID: 17989089 8. Harms, FL. et al. (2018) American journal of human genetics 103, 579-591. PMID: 30290153 9. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447 10. Brown, JL. et al. (1996) Current biology : CB 6, 598-605. PMID: 8805275 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human PAK1 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
