Anti-PAK1 Polyclonal Antibody (HC494014)

Name: Anti-PAK1 Polyclonal Antibody
Brand: abinScience
SKU: HC494014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC494014

Description

Anti-PAK1 Polyclonal Antibody (HC494014) is a rabbit polyclonal antibody detecting PAK1 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PAK1 (Glu252-Cys501).

Target

Alpha-PAK, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, PAK1

Purification

Purified by antigen affinity column.

Accession

Q13153

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase PAK 1 (PAK1) is a ~60 kDa protein. Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases.

1. Zenke, FT. et al. (1999) The Journal of biological chemistry 274, 32565-73. PMID: 10551809

2. Manabe, R. et al. (2002) Journal of cell science 115, 1497-510. PMID: 11896197

3. Slack-Davis, JK. et al. (2003) The Journal of cell biology 162, 281-91. PMID: 12876277

4. Zhou, GL. et al. (2003) Molecular and cellular biology 23, 8058-69. PMID: 14585966

5. Zhou, H. et al. (2005) The Journal of biological chemistry 280, 10624-35. PMID: 15611088

6. Rider, L. et al. (2007) The Journal of biological chemistry 282, 30985-96. PMID: 17726028

7. Mayhew, MW. et al. (2007) Journal of cell science 120, 3911-8. PMID: 17989089

8. Harms, FL. et al. (2018) American journal of human genetics 103, 579-591. PMID: 30290153

9. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447

10. Brown, JL. et al. (1996) Current biology : CB 6, 598-605. PMID: 8805275

Note

For research use only.

Images

References

Datasheet

Anti-PAK1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PAK1 Polyclonal Antibody (HC494014)

Overview

Images

References

Datasheet

SDS

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