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| カタログ番号 | HS727012 |
|---|---|
| 説明 |
Recombinant Human PARP14 Protein, N-His (HS727012) expressed in E. coli, spanning Ala788-Leu1194. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q460N5 |
| タンパク質長 | Ala788-Leu1194 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 46.25 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ADP-ribosyltransferase diphtheria toxin-like 8, ARTD8, B aggressive lymphoma protein 2, BAL2, EC:2.4.2.-, KIAA1268, PARP-14, PARP14, Poly [ADP-ribose] polymerase 14, Protein mono-ADP-ribosyltransferase PARP14 |
| 背景 | Protein mono-ADP-ribosyltransferase PARP14 is a ~202 kDa protein. ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation. However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703'. Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription. 1. Aguiar, RC. et al. (2005) The Journal of biological chemistry 280, 33756-65. PMID: 16061477 2. Kleine, H. et al. (2008) Molecular cell 32, 57-69. PMID: 18851833 3. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379 4. Iwata, H. et al. (2016) Nature communications 7, 12849. PMID: 27796300 5. Begitt, A. et al. (2018) Nature communications 9, 2144. PMID: 29858569 |
| 注意事項 | For research use only |
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