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Human PEX5 Recombinant Protein (N-His) (HW731012)

Human PEX5 Recombinant Protein (N-His)
Human PEX5 Recombinant Protein (N-His)
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  • 50ug
  • 100ug
  • 1mg
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Aperçu
Numéro de catalogueHW731012
Description
Recombinant Human PEX5 Protein, N-His (HW731012) expressed in E. coli, spanning His306-Gln639. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsP50542
Longueur de protéineHis306-Gln639
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 39.26 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsPeroxisomal targeting signal 1 receptor, PTS1 receptor, PTS1-BP, PTS1R, Peroxin-5, Peroxisome receptor 1, PXR1, PEX5, Peroxisomal C-terminal targeting signal import receptor
Contexte

Peroxisomal targeting signal 1 receptor (PEX5/PTS1) is a ~70 kDa protein. Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.

1. Gatto, GJ. et al. (2000) Nature structural biology 7, 1091-5. PMID: 11101887
2. Dammai, V. et al. (2001) Cell 105, 187-96. PMID: 11336669
3. Harper, CC. et al. (2003) The Journal of biological chemistry 278, 7897-901. PMID: 12456682
4. Miyata, N. et al. (2005) Molecular and cellular biology 25, 10822-32. PMID: 16314507
5. Stanley, WA. et al. (2006) Molecular cell 24, 653-663. PMID: 17157249
6. Stanley, WA. et al. (2007) BMC structural biology 7, 24. PMID: 17428317
7. Freitas, MO. et al. (2011) The Journal of biological chemistry 286, 40509-19. PMID: 21976670
8. Zhang, J. et al. (2015) Nature cell biology 17, 1259-1269. PMID: 26344566
9. Fransen, M. et al. (1995) The Journal of biological chemistry 270, 7731-6. PMID: 7706321
10. Dodt, G. et al. (1995) Nature genetics 9, 115-25. PMID: 7719337
Note For research use only.
Images
  • Human PEX5 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human PEX5 Protein, N-His

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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