Anti-Human PEX5 Polyclonal Antibody (HW731014)

Name: Anti-Human PEX5 Polyclonal Antibody
Brand: abinScience
SKU: HW731014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW731014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PEX5 (His306-Gln639).

Target

Peroxisomal targeting signal 1 receptor, PTS1 receptor, PTS1-BP, PTS1R, Peroxin-5, Peroxisome receptor 1, PXR1, PEX5, Peroxisomal C-terminal targeting signal import receptor

Purification

Purified by antigen affinity column.

Accession

P50542

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peroxisomal targeting signal 1 receptor (PEX5/PTS1) is a ~70 kDa protein. Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.

1. Gatto, GJ. et al. (2000) Nature structural biology 7, 1091-5. PMID: 11101887

2. Dammai, V. et al. (2001) Cell 105, 187-96. PMID: 11336669

3. Harper, CC. et al. (2003) The Journal of biological chemistry 278, 7897-901. PMID: 12456682

4. Miyata, N. et al. (2005) Molecular and cellular biology 25, 10822-32. PMID: 16314507

5. Stanley, WA. et al. (2006) Molecular cell 24, 653-663. PMID: 17157249

6. Stanley, WA. et al. (2007) BMC structural biology 7, 24. PMID: 17428317

7. Freitas, MO. et al. (2011) The Journal of biological chemistry 286, 40509-19. PMID: 21976670

8. Zhang, J. et al. (2015) Nature cell biology 17, 1259-1269. PMID: 26344566

9. Fransen, M. et al. (1995) The Journal of biological chemistry 270, 7731-6. PMID: 7706321

10. Dodt, G. et al. (1995) Nature genetics 9, 115-25. PMID: 7719337

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PEX5 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Mouse brain lysate
Lane 3: Rat brain lysate

References

Datasheet

Anti-Human PEX5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human PEX5 Polyclonal Antibody (HW731014)

Overview

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References

Datasheet

SDS

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