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| カタログ番号 | HP991012 |
|---|---|
| 説明 |
Recombinant Human PIKFYVE Protein, N-His (HP991012) expressed in E. coli, spanning Ala1864-Cys2098. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9Y2I7 |
| タンパク質長 | Ala1864-Cys2098 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 29.32 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Serine-protein kinase PIKFYVE, Phosphatidylinositol 3-phosphate 5-kinase, PIKfyve, 1-phosphatidylinositol 3-phosphate 5-kinase, PIKFYVE, FYVE finger-containing phosphoinositide kinase, Type III PIP kinase, PIPkin-III, KIAA0981, PIP5K3, Phosphatidylinositol 3-phosphate 5-kinase type III |
| 背景 | 1-phosphatidylinositol 3-phosphate 5-kinase (PIKFYVE) is a ~237 kDa protein. Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression. The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form (PtdIns(3,5)P2). Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5-phosphate (PtdIns(5)P). Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate. 1. Shisheva, A. (2012) Current topics in microbiology and immunology 362, 127-62. PMID: 23086417 2. Sbrissa, D. et al. (2007) The Journal of biological chemistry 282, 23878-91. PMID: 17556371 3. Sbrissa, D. et al. (2012) American journal of physiology. Cell physiology 303, C436-46. PMID: 22621786 4. Lees, JA. et al. (2020) Molecular cell 80, 736-743.e4. PMID: 33098764 5. Baranov, MV. et al. (2019) iScience 11, 160-177. PMID: 30612035 6. Krishna, S. et al. (2016) Developmental cell 38, 536-47. PMID: 27623384 7. Dayam, RM. et al. (2017) Journal of immunology (Baltimore, Md. : 1950) 199, 2096-2105. PMID: 28779020 8. Liggins, MC. et al. (2018) PLoS genetics 14, e1007290. PMID: 29584722 9. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human PIKFYVE Protein, N-His
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