Anti-Human PIKFYVE Polyclonal Antibody (HP991014)

Name: Anti-Human PIKFYVE Polyclonal Antibody
Brand: abinScience
SKU: HP991014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP991014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PIKFYVE (Ala1864-Cys2098).

Target

Serine-protein kinase PIKFYVE, Phosphatidylinositol 3-phosphate 5-kinase, PIKfyve, 1-phosphatidylinositol 3-phosphate 5-kinase, PIKFYVE, FYVE finger-containing phosphoinositide kinase, Type III PIP kinase, PIPkin-III, KIAA0981, PIP5K3, Phosphatidylinositol 3-phosphate 5-kinase type III

Purification

Purified by antigen affinity column.

Accession

Q9Y2I7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

1-phosphatidylinositol 3-phosphate 5-kinase (PIKFYVE) is a ~237 kDa protein. Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression. The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form (PtdIns(3,5)P2). Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5-phosphate (PtdIns(5)P). Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate.

1. Shisheva, A. (2012) Current topics in microbiology and immunology 362, 127-62. PMID: 23086417

2. Sbrissa, D. et al. (2007) The Journal of biological chemistry 282, 23878-91. PMID: 17556371

3. Sbrissa, D. et al. (2012) American journal of physiology. Cell physiology 303, C436-46. PMID: 22621786

4. Lees, JA. et al. (2020) Molecular cell 80, 736-743.e4. PMID: 33098764

5. Baranov, MV. et al. (2019) iScience 11, 160-177. PMID: 30612035

6. Krishna, S. et al. (2016) Developmental cell 38, 536-47. PMID: 27623384

7. Dayam, RM. et al. (2017) Journal of immunology (Baltimore, Md. : 1950) 199, 2096-2105. PMID: 28779020

8. Liggins, MC. et al. (2018) PLoS genetics 14, e1007290. PMID: 29584722

9. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029

Note

For research use only.

Images

References

Datasheet

Anti-Human PIKFYVE Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human PIKFYVE Polyclonal Antibody (HP991014)

Overview

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References

Datasheet

SDS

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