Human PIM1 Recombinant Protein (N-His) (HY563012)

Name: Human PIM1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY563012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HY563012
Descripción	Recombinant Human PIM1 Protein, N-His (HY563012) expressed in E. coli, spanning Ser98-Lys313. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P11309
Longitud de proteína	Ser98-Lys313
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	27.52 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	PIM1, Serine/threonine-protein kinase pim-1
Fondo	Serine/threonine-protein kinase pim-1 (PIM1) is a ~35 kDa protein. Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. 1. Stout, BA. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 6409-17. PMID: 15528381 2. Saris, CJ. et al. (1991) The EMBO journal 10, 655-64. PMID: 1825810 3. Padi, SKR. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 20505-20510. PMID: 31548394 4. Morishita, D. et al. (2008) Cancer research 68, 5076-85. PMID: 18593906 5. Gu, JJ. et al. (2009) Oncogene 28, 4261-71. PMID: 19749799 6. Bachmann, M. et al. (2006) The international journal of biochemistry & cell biology 38, 430-43. PMID: 16356754 7. Wang, Z. et al. (2002) Biochimica et biophysica acta 1593, 45-55. PMID: 12431783 8. Koike, N. et al. (2000) FEBS letters 467, 17-21. PMID: 10664448 9. Fisch, D. et al. (2023) Science (New York, N.Y.) 382, eadg2253. PMID: 37797010
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human PIM1 Protein, N-His

Referencias

Datasheet

Human PIM1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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