Anti-PIM1 Polyclonal Antibody (HY563014)

Name: Anti-PIM1 Polyclonal Antibody
Brand: abinScience
SKU: HY563014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HY563014

Description

Anti-PIM1 Polyclonal Antibody (HY563014) is a rabbit polyclonal antibody detecting PIM1 in ELISA, IHC, WB. Suitable for Bovine, Cat, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PIM1 (Ser98-Lys313).

Target

PIM1, Serine/threonine-protein kinase pim-1

Purification

Purified by antigen affinity column.

Accession

P11309

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase pim-1 (PIM1) is a ~35 kDa protein. Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1.

1. Stout, BA. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 6409-17. PMID: 15528381

2. Saris, CJ. et al. (1991) The EMBO journal 10, 655-64. PMID: 1825810

3. Padi, SKR. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 20505-20510. PMID: 31548394

4. Morishita, D. et al. (2008) Cancer research 68, 5076-85. PMID: 18593906

5. Gu, JJ. et al. (2009) Oncogene 28, 4261-71. PMID: 19749799

6. Bachmann, M. et al. (2006) The international journal of biochemistry & cell biology 38, 430-43. PMID: 16356754

7. Wang, Z. et al. (2002) Biochimica et biophysica acta 1593, 45-55. PMID: 12431783

8. Koike, N. et al. (2000) FEBS letters 467, 17-21. PMID: 10664448

9. Fisch, D. et al. (2023) Science (New York, N.Y.) 382, eadg2253. PMID: 37797010

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PIM1 polyclonal antibody at 1ug/mL on recombinant PIM1 (Catalog No. HY563012).

References

Datasheet

Anti-PIM1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PIM1 Polyclonal Antibody (HY563014)

Overview

Images

References

Datasheet

SDS

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